ASCEN Pre Stained Morphology Slide Intended use:
to assess the morphological parmeters of cells such as spermatozoa.
The stained cells can be assessed with them help of an automated
system(i.e. CASA) or manually
Principle of the device:
when a small volume of cells / semen is deposited on a pre-stained
slide,the fixative-stain film dissolves in fluid/seminal plasma and
stains (sperm) cells
Preparations before use:
Remove ASCEN pre-stained morphology slide from packaging and take
care not to touch the coated area. Make sure the coated area is
undamaged. Access toan incubator at 55°C / 131F, or an ethanol flame. Use semen after
liquefaction or (sperm) sample in a culture medium
*Check the color stained on surface before add on sample
*Pipette 5l of sample on the endside of the slide prepare the smear
like figure 1
*Put the slide on the hotplate 56°C 30 -60 seconds
*Drop the slide into the water jar 1 second
*Dry the slide in hotplate
*Start assessment immediately after
Automatic analyzing -use SCA sperm morph.
System In order to improve the efficiency of the accuracy of the
We recommend to wash the sample .
1.mix gently of 0.2~0.5ml (relate with the density of sperm)
liquefaction semen with 10ml physiological saline or 10ml PBS
2.centrifugal 10min 800g
3.removing most of the supernatant
4.Mix gently the rest, usually a 20~40l.
5.count the concentration of sperm
1)= 45M/ml,perfect go to Staining procedure
2)< 45M/ml,add 20ul physiological saline or 10ml PBS (pH7.40.2)
repeat step 2