Pre-Stained Morphology Slides For Sperm Morphology Analysis

Pre-Stained Morphology Slides For Sperm Morphology Analysis

• name：Pre-Stained Morphology Slides
• usage：sperm morphology analysis
• apply：CASA Semen Analysis
• Approval：CE FDA
• Product description: Pre-Stained Morphology Slides For Sperm Morphology Analysis
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ASCEN Pre Stained Morphology Slide Intended use:

to assess the morphological parmeters of cells such as spermatozoa. The stained cells can be assessed with them help of an automated system(i.e. CASA) or manually

Principle of the device:

when a small volume of cells / semen is deposited on a pre-stained slide,the fixative-stain film dissolves in fluid/seminal plasma and stains (sperm) cells

Preparations before use:

Remove ASCEN pre-stained morphology slide from packaging and take care not to touch the coated area. Make sure the coated area is undamaged. Access toan incubator at 55°C / 131F, or an ethanol flame. Use semen after liquefaction or (sperm) sample in a culture medium

Staining Procedure：

*Check the color stained on surface before add on sample

*Pipette 5l of sample on the endside of the slide prepare the smear like figure 1

*Put the slide on the hotplate 56°C 30 -60 seconds

*Drop the slide into the water jar 1 second

*Dry the slide in hotplate

*Start assessment immediately after

Automatic analyzing -use SCA sperm morph.

System In order to improve the efficiency of the accuracy of the sperms identification

We recommend to wash the sample .

Wash procedure

1.mix gently of 0.2~0.5ml (relate with the density of sperm) liquefaction semen with 10ml physiological saline or 10ml PBS (pH7.40.2)

2.centrifugal 10min 800g

3.removing most of the supernatant

4.Mix gently the rest, usually a 20~40l.

5.count the concentration of sperm

1)= 45M/ml,perfect go to Staining procedure

2)< 45M/ml,add 20ul physiological saline or 10ml PBS (pH7.40.2) repeat step 2

3)> 45M/ml,dilute