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Laboratory Porcine ELISA Kit , Erythropoietin EPO ELISA Kit CE / ISO / MSDS Certificated

Categories Porcine ELISA Kit
Brand Name: BT Lab
Model Number: Cat.No E0429Po
Certification: CE, ISO9001:2005, MSDS
Place of Origin: Shanghai, China
MOQ: 1 kit
Price: Negotiation
Payment Terms: Western Union, T/T
Supply Ability: In Stock
Delivery Time: 1-3 business days, bulk order within one week
Packaging Details: Wrapped with ice pack and styrofoam package
Target Protein: Erythropoietin
Sample: serum,plasma,urine,tissue,cell culture supernatant
Assay Time: 2 hours
Discount: Available
Brand: BT Lab
Assay Principle: Sandwich
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Laboratory Porcine ELISA Kit , Erythropoietin EPO ELISA Kit CE / ISO / MSDS Certificated

Porcine Erythropoietin ELISA Kit with High Spesificity and Sensitivity

Cat.No E0429Po


Reagent Provided

ComponentsQuantity

Standard Solution (2400ng/L)

0.5ml x1
Pre-coated ELISA Plate12 * 8 well strips x1
Standard Diluent3ml x1
Streptavidin-HRP6ml x1
Stop Solution6ml x1
Substrate Solution A6ml x1
Substrate Solution B6ml x1
Wash Buffer Concentrate (30x)20ml x1
Biotinylated Porcine EPO Antibody1ml x1
User Instruction1
Plate Sealer2 pics
Zipper bag1 pic

Specimen Collection

Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.


Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.


Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


Product Tags:

protein elisa kit

  

elisa test kit

  
Quality Laboratory Porcine ELISA Kit , Erythropoietin EPO ELISA Kit CE / ISO / MSDS Certificated for sale
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